No cell culture problem is as universal as that of culture loss due to contamination. All cell culture laboratories and cell culture workers have experienced it.
Culture contaminants may be biological or chemical, seen or unseen, destructive or seemingly benign, but in all cases they adversely affect both the use of your cell cultures and the quality of your research.
If your lab have been found in this situation, take a look this magnific guide to Understanding and Managing Cell Culture Contamination
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